Biology Agriculture Aquaculture Horticulture Postharvest Forestry Geography Environment Health





Molecular diagnosis of mango malformation disease and phylogeny of Fusarium mangiferae

submitted by ox451apcndr 2 years and 5 months ago
Mango malformation disease (MMD) caused by various Fusaria, including the fungus Fusarium mangiferae, is difficult to diagnose and cannot be controlled effectively. In this study a PCR diagnostic tool was developed for detection of F. mangiferae by generating primers which flank fungus-specific sequences. The assay is suitable for both in-vitro and in-vivo tests, and is capable of detecting 1 pg of the fungal DNA. Relatively high genetic variation was discovered between various isolates of the pathogen based on amplified fragment length polymorphism (AFLP) analyses. In addition, some isolates of F. mangiferae (including the accession numbers FMS-123 and NRRL 25226), were found to be quite distinct from most F. mangiferae accessions. Likewise, representative MMD-associated Fusaria from the Asian and American clades of the Gibberella fujikuroi species complex were further differentiated by AFLP analyses.


Topic: Agriculture

Add your comment

Please Login or Signup to leave a comment

Related stories

Mango Malformation Disease in South China Caused by Fusarium proliferatum
submitted by sallinmaan 4 years and 4 months ago - Topic: Biology
A total of 13 Fusarium isolates were obtained from samples of malformed mango seedlings from Yunnan and Sichuan provinces in China, and five morphologically similar isolates were confirmed causing the disease by satisfying Koch's postulates. One typical isolate (MG4) was selected for detailed morphological and molecular studies. Based on the following morphological characteristics, isolate MG4 ...

Molecular diagnosis and phylogenetic analysis of Babesia bigemina and Babesia bovis hemoparasites from cattle in South Africa
submitted by rmocep 1 year and 1 month ago - Topic: Agriculture
Babesia parasites, mainly Babesia bovis and B. bigemina, are tick-borne hemoparasites inducing bovine babesiosis in cattle globally. The clinical signs of the disease include, among others, anemia, fever and hemoglobinuria. Babesiosis is known to occur in tropical and subtropical regions of the world. In this study, we aim to provide information about the occurrence and phylogenetic relationshi...



A molecular diagnosis method using real-time PCR for quantification and detection of Fusarium oxysporum f. sp. cubense race 4
submitted by ondriomongane 1 year and 10 months ago - Topic: Agriculture
The Fusarium genus causes devastating plant diseases worldwide, in which Fusarium oxysporum is the most serious crop pathogen. Disease monitoring is the basis of integrated pest management of any disease. The lack of rapid, accurate, and reliable device to detect and identify plant pathogens is one of the main limitations in integrated disease management. This study describes an efficient and q...

Cutaneous Fusariosis by a species of the Fusarium dimerum species complex in a Patient with Acute Mieloblastic Leukemia
submitted by betes 1 year and 8 months ago - Topic: Biology
Fusariosis is an emergent opportunistic hialohyphomycosis produced by fungi belonging to the genus Fusarium. These moulds are capable of producing life-threatening diseases in immunocompromised hosts, especially in those suffering from leukemia. It has also been described in immunocompetent patients, where it usually causes non-invasive localized lesions. Fusariosis in immunocompromised individ...


Impaired innate interferon induction in severe therapy resistant atopic asthmatic children
submitted by mofotlolmkhir 1 year and 7 months ago - Topic: Health
Deficient type I interferon-β and type III interferon-λ induction by rhinoviruses has previously been reported in mild/moderate atopic asthmatic adults. No studies have yet investigated if this occurs in severe therapy resistant asthma (STRA). Here, we show that compared with non-allergic healthy control children, bronchial epithelial cells cultured ex vivo from severe t...

Molecular phylogeny and pathotyping of Fusarium solani: a causal agent of Dalbergia sissoo decline
submitted by babmellir2059 1 year and 1 month ago - Topic: Forestry
Sissoo (Dalbergia sissoo), commonly known as shisham, is amongst the finest woods of South Asia, but ‘wilt’ disease has caused a rapid decline in this species. The cause of the disease remains uncertain. The aim of this study is to identify the causal agent of the disease and characterize isolates made from diseased trees, based on genomic data and variations in virulence. Samples of infect...


Caroli Disease Associated With Vein of Galen Malformation in a Male Child
submitted by vondzangg 1 month ago - Topic: Health
We report the first case of a male child with both Caroli disease and vein of Galen malformation. The neonate presented to our department with congestive heart failure as a result of the intracranial arteriovenous high-flow shunt. Over time, several endovascular embolizations led to a complete angiographic occlusion of the shunt. Additionally, the diagnosis of Caroli disease was made at the age...

Refractory disseminated Fusariosis by Fusarium verticillioides in a patient with acute myeloid leukaemia relapsed after allogeneic hematopoietic stem cell transplantation: a case report and literature review
submitted by pitirclorki 2 years and 2 months ago - Topic: Biology
Fusarium spp. are among the leading fungal pathogens to cause invasive mould infections in patients with hematopoietic malignancy. The Fusarium spp. most frequently involved in human infections are Fusarium solani, Fusarium oxysporum and Fusarium verticillioides. However, identification is a cumbersome and time-consuming task. Fusarium spp. are resistant “in vitro” to many of antifungal age...


Comparison between Aniridia with and without PAX6 Mutations Clinical and Molecular Analysis in 14 Korean Patients with Aniridia
submitted by wonnybanny 2 years and 2 months ago - Topic: Health
To describe clinical and molecular characteristics of Korean patients with aniridia and to compare the clinical phenotype between those having an identifiable PAX6 mutation and those not. Comparative case series. A total of 14 Korean patients from 1 families with aniridia. Complete ophthalmologic examinations were performed for all patients. PAX6 analysis included direct sequencing of all codin...

Novel mutations in the MNX1 gene in two families with Currarino syndrome and variable phenotype
submitted by tozzaka 2 years and 1 month ago - Topic: Biology
The Currarino syndrome (CS) consists of a sacral defect, an anorectal malformation and a pre-sacral mass. It manifests as an autosomal dominant congenital malformation in familial settings, with varying penetrance. The disease-causing gene, Motor neuron and pancreas homeobox-1 (MNX1), is known to be mutated in almost all familial cases, but due to the lack of genotype–phenotype correlation, t...