Effect of titanium surface topographies on human bone marrow stem cells differentiation in vitro

Science Alerts Social Network

	View All Agriculture Biology Environment Forestry Geography Health

Effect of titanium surface topographies on human bone marrow stem cells differentiation in vitro

submitted by rozon 11 months ago

Coating characteristics of dental implants such as composition and topography regulate cell response during implant healing. The aim of this study was to assess how surface topography can affect osteogenic differentiation of mesenchymal stem cells (MSCs) by analyzing the expression levels of bone-related genes and MSCs marker. Thirty disk-shaped, commercially pure Grade 2 titanium samples (1 × 2 mm) with 3 different surface topographies (DENTSPLY-Friadent GmbH, Mannheim, Germany) were used in the present study: 1 Ti machined disks (control), 1 Ti sandblasted and acid-etched disks (DPS^®) and 1 sandblasted and acid-etched disks at high temperature (Plus^®). Samples were processed for real-time reverse transcription-polymerase chain reaction (RT-PCR) analysis. By comparing machined and Plus^® disks, quantitative real-time RT-PCR showed a significant reduction of the bone-related genes osteocalcin (BGLAP) and osteoblast transcriptional factor (RUNX2). The comparison between DPS^® and Plus^® disks showed a slight induction of all the genes examined (RUNX2, ALPL, COL1A1, COL3A1, ENG, FOSL1, SPP1, and SP7); only the expression of BGLAP remained stable. The present study, demonstrated that implant surface topography affects osteoblast gene expression. Indeed, Plus^® surface produces an effect on MSCs in the late differentiation stages.

Topic: Health

Add your comment

Please Login or Signup to leave a comment

Related stories

Chondrogenic differentiation of human subchondral progenitor cells is affected by synovial fluid from donors with osteoarthritis or rheumatoid arthritis

submitted by erfon241 11 months ago - Topic: Health

Microfracture is a first-line treatment option for cartilage repair. In microfracture, subchondral mesenchymal cortico-spongious progenitor cells (CSP) enter the defect and form cartilage repair tissue. The aim of our study was to investigate the effects of joint disease conditions on the in vitr...

Differential effect of platelet-rich plasma and fetal calf serum on bone marrow-derived human mesenchymal stromal cells expanded in vitro

submitted by aloisso3119 2 years and 4 months ago - Topic: Health

Mesenchymal stromal cells (MSCs) derived from various sources have great potential for use in cell-based therapies. Since the proportion of primary MSCs contained in bone marrow or adipose tissue is low, plastic adherence and in vitro expansion are necessary to expand MSCs prior to clinical appli...

Effects of transforming growth factor-β subtypes on in vitro cartilage production and mineralization of human bone marrow stromal-derived mesenchymal stem cells

submitted by mawo531 2 years and 3 months ago - Topic: Health

Human bone marrow stromal-derived mesenchymal stem cells (hBMSCs) will differentiate into chondrocytes in response to defined chondrogenic medium containing transforming growth factor-β (TGFβ). in the literature suggest that the three mammalian subtypes of TGFβ (TGFβ1, TGFβ2 and TGFβ3) prov...

Osteogenic differentiation of GFP-labeled human umbilical cord blood derived mesenchymal stem cells after cryopreservation

submitted by mitiyir 1 year and 11 months ago - Topic: Biology

The osteogenic capacity of human umbilical cord blood derived mesenchymal stem cells (UCB-MSCs) has been demonstrated both in vitro and in vivo. Therefore, cell labeling and storage are becoming necessary for researching the potential therapeutic use of UCB-MSCs for bone tissue engineering. The a...

Effect of a structurally modified human granulocyte colony stimulating factor, G-CSFa, on leukopenia in mice and monkeys

submitted by printpok 1 year and 11 months ago - Topic: Health

Granulocyte colony stimulating factor (G-CSF) regulates survival, proliferation, and differentiation of neutrophilic granulocyte precursors, Recombinant G-CSF has been used for the treatment of congenital and therapy-induced neutropenia and stem cell mobilization. Due to its intrinsic instability...

Clonal multipotency and effect of long-term in vitro expansion on differentiation potential of human hair follicle derived mesenchymal stem cells

submitted by jdenh 1 year and 9 months ago - Topic: Biology

Hair follicle harbors a rich stem cell pool with mesenchymal lineage differentiation potential. Although previous studies with rodent cells demonstrated that hair follicle sheath and papilla cells possess multi-lineage differentiation potential, human hair follicle derived mesenchymal stem cells ...

Rac1b Regulates NT3-Stimulated Mek-Erk Signaling, Directing Marrow-Isolated Adult Multilineage Inducible (MIAMI) Cells Toward an Early Neuronal Phenotype

submitted by jwoezil 1 year and 6 months ago - Topic: Health

Due to the limitations of neural stem cells to repair neuronal damage in the human brain, alternative approaches of repair using autologous adult stem cells have been examined for direct cell-replacement, or paracrine mediated neuroprotective effects. Human bone marrow–derived stromal cells (hM...

The significance of differential expression of genes and proteins in human primary cells caused by microgrooved biomaterial substrata

submitted by trichas3111 1 year and 3 months ago - Topic: Biology

We demonstrate that etched microgrooves, with truncated V-shape in cross-section and subsequent acid etching, on titanium substrata alter the expression of various genes and proteins in human primary cells. Etched microgrooves with 3 or 6 μm width and 1 μm depth promoted human gingival fibrob...

Diverse effects of type II collagen on osteogenic and adipogenic differentiation of mesenchymal stem cells

submitted by oprenciss61 1 year and 2 months ago - Topic: Biology

Type II collagen is known to modulate chondrogenesis of mesenchymal stem cells (MSCs). In this study, MSCs from human bone marrow aspirates were used to study the modulating effects of type II collagen on MSC differentiation during the early stages of osteogenesis and adipogenesis. With osteogeni...

Effetti della low-level laser irradiation su cellule staminali mesenchimali inoculate in una biomatrice tridimensionale: studio pilota in vitro

submitted by qayhap2080 5 months ago - Topic: Health

Lo scopo di questo studio era valutare l’effetto del laser Nd:Yag sulla proliferazione e sulla differenziazione di cellule staminali mesenchimali (MSC) indotte verso la linea osteoblastica. Le MSC sono state prelevate da midollo osseo umano adulto, isolate e piastrate in mezzo completo (α-MEM)...

PTH induces differentiation of mesenchymal stem cells by enhancing BMP signaling

submitted by kiyg 1 year and 1 month ago - Topic: Health

Parathyroid hormone (PTH) stimulates bone remodeling and induces differentiation of bone marrow mesenchymal stromal/stem cells (MSCs) by orchestrating activities of local factors such as bone morphogenetic proteins (BMPs). The activity and specificity of different BMP ligands are controlled by va...