Biology Agriculture Aquaculture Horticulture Postharvest Forestry Geography Environment Health

Biocontrol potential of an endophytic Bacillus pumilus JK-SX001 against Poplar Canker

submitted by safeo 9 months ago
Several endophytes play a vital role in plant protection and plant growth promotion. In China, poplar canker disease caused by three pathogens (Cytospora chrysosperma, Phomopsis macrospora and Fusicoccum aesculi) is difficult to control by use of chemicals; therefore, use of biocontrol agents is desirable. The aim of this study was to examine the effects of strain JK-SX001 on plant growth and poplar canker caused by three pathogens. It is clearly demonstrated that strain JK-SX001 described as Bacillus pumilus by the Biolog identification system combined with morphological, physiological and biochemical tests and phylogenetic (16S rDNA) studies. Strain JK-SX001 produced lytic enzymes such as cellulases and protease. In vitro antagonistic assays showed that secondary metabolites of JK-SX001 extracted using methylbenzene could suppress the growth of three pathogens effectively. A derivative of this strain, labeled with gfp, was also used to study the colonization of its poplar hosts. This strain was verified as an endophytic bacterium of poplar by confocal laser scanning microscopy. The population of the GFP-labeled JK-SX001 inoculant was larger and more stable in roots and stems than that in leaves. The evaluation of the antagonistic strains against poplar canker indicated that JK-SX001 effectively reduced disease incidence. In greenhouse studies, poplar seedlings inoculated with this strain showed high increase in biomass production, shoot length, stem diameter and photosynthetic activity. Strain JK-SX001 colonized poplar roots, stems and leaves endophytically, promoting plant growth and suppressing pathogenic activities on seedling of poplar. Our results demonstrate JK-SX001 as a promising candidate for biocontrol of poplar canker. Symptoms of Poplar seedlings inoculated with three pathogens. 1 Inoculated with Cytospora chrysosperma, 2 Inoculated with Phomopsis macrospore, 3 Inoculated with Fusicoccum aesculi

Topic: Agriculture

Add your comment

Please Login or Signup to leave a comment

Related stories

Use of Biocontrol Organisms and Compost Amendments for Improved Control of Soilborne Diseases and Increased Potato Production
submitted by sasonstontan 1 year and 5 months ago - Topic: Agriculture
Soilborne potato diseases are persistent problems in potato production and alternative management practices are needed. In this research, biocontrol agents (Bacillus subtilis GB03 and Rhizoctonia solani hypovirulent isolate Rhs1A1) and compost amendments (from different source material), were evaluated alone and in combination, for their potential to reduce soilborne diseases and increase tuber...

Biocontrol bacterial communities associated with diseased peanut (Arachis hypogaea L.) plants
submitted by tormik97 1 year and 10 months ago - Topic: Biology
In the present study, the isolation and characterization of endophytic and epiphytic biocontrol bacteria from peanut plants affected with white mold and root rot are described. Two hundred and sixty three isolates were selected from diseased plants and biocontrol traits analyzed were antibiosis against Sclerotinia minor and Fusarium solani, siderophore production and similarities in carbon sour...

Parasitic Bacteria and Fungi on Common Mistletoe (Viscum album L.) and Their Potential Application in Biocontrol
submitted by oot 1 year and 8 months ago - Topic: Health
This study was carried out to identify pathogenic bacteria and fungi on mistletoe (Viscum album L.) and investigate their potential use in biological control of this parasitic plant. For this purpose, a total of 48 fungal isolate and 193 bacterial strains were isolated from contaminated V. album during the summers 2005–2006. The isolated bacterial strains and fungal isolates were identified ...

Application of endophytic bacteria for controlling anthracnose disease (Colletotrichum lindemuthianum) on bean plants
submitted by csogimd 1 year and 2 months ago - Topic: Agriculture
Over 100 endophytic bacterial isolates were isolated from surface-sterilised roots of the Fabaceae family in East Azerbaijan farms. These isolates were screened for their in vitro biocontrol activity against Colletotrichum lindemuthianum by dual culture technique using potato dextrose agar (PDA) medium. Eight bacterial isolates (Bacillus subtilis subsp. subtilis, Bacillus atrophaeus, B. tequile...

Biocontrol activity of Bacillus against a GFP-marked Pseudomonas syringae pv. tomato on tomato phylloplane
submitted by soltcido 1 year and 1 month ago - Topic: Agriculture
We report the biocontrol activity of the endophytic bacteria Bacillus pumilus and Bacillus amyloliquefacies against the plant pathogenic bacterium Pseudomonas syringae pv. tomato strain NS4 transformed with the GFP expressing gene. P. s. pv. tomato strain NS4 was obtained from the transformation of P. s. pv. tomato wild-type strain NW with the plasmid pNKGFP containing GFP-cassette for chromoso...

Identification and evaluation of a potential biocontrol agent, Bacillus subtilis, against Fusarium sp. in apple seedlings
submitted by roze 1 year and 1 month ago - Topic: Biology
To find a potential biocontrol agent against Fusarium sp. in apple seedlings, an endophytic bacterium strain was isolated from apple tree tissues. The inhibitive efficiency of the isolated strain against the hyphal growth of Fusarium sp. and Rhizoctonia solani was tested. Strain Y-1 showed significant inhibitory effects against Fusarium oxysporum, F. moniliforme, F. proliferatum, F. solani and ...

Probiotic Strains for Shellfish Aquaculture: Protection of Eastern Oyster, Crassostrea virginica, Larvae and Juveniles Againsl Bacterial Challenge
submitted by dlob77 10 months ago - Topic: Biology
Bacterial pathogens, including several Vibrio spp. and Roseovarius crassostreae, cause severe mortality of larval and juvenile eastern oysters. The introduction of beneficial bacterial isolates in oyster hatcheries and nurseries for the biocontrol of bacterial diseases is a good alternative to the use of antibiotics. The goal of this study was to screen and characterize marine bacterial isolate...

Rhizosphere and endophytic bacteria for the suppression of eggplant wilt caused by Ralstonia solanacearum
submitted by bolzonmv 2 years and 3 months ago - Topic: Agriculture
Forty eight endophytic bacteria and 11 rhizobacteria were screened for their antibacterial activity against Ralstonia solanacearum, causal agents of eggplant wilt. Among 22 effective antagonistic isolates, 18 were Pseudomonas spp. forming three groups based on biochemical characterization. Talc formulation of the antagonistic bacteria and non-formulated 24 h old grown antagonistic bacterial ce...

Rapeseed rotation, compost and biocontrol amendments reduce soilborne diseases and increase tuber yield in organic and conventional potato production systems
submitted by ogradivca 9 months ago - Topic: Agriculture
Integrating multiple soil and disease management practices may improve crop productivity and disease control, but potential interactions and limitations need to be determined.Three different potential disease-suppressive management practices, including a Brassica napus (rapeseed) green manure rotation crop, conifer-based compost amendment, and three biological control organisms (Trichoderma vir...

Identification and Characterisation of New Microbial Antagonists for Biocontrol of Monilinia laxa, the Causal Agent of Brown Rot on Stone Fruit
submitted by oyb119 9 months ago - Topic: Agriculture
Monilinia laxa is the causal agent of brown rot disease on stone fruits, and also causes blossom wilt and twig canker. The common practice used to manage this disease is through fungicide treatments. However the demand to reduce fungicide inputs has been increasing and there is a growing number of reports of M. laxa strains that are resistant to fungicides. There is an urgent need to search for...

Plant growth promoting activity of Bacillus pumilus in tea (Camellia sinensis) and its biocontrol potential against Poria hypobrunnea
submitted by eyoda86 6 months ago - Topic: Agriculture
Bacillus pumilus TRS-3, isolated from tea rhizosphere, was evaluated for its plant growth promoting and biocontrol activities both in vitro and in vivo. The bacterium showed positive PGPR traits in vitro such as phosphate solubilization, siderophore production, volatile production and IAA secretion. B. pumilus inhibited growth of tea root fungal pathogens such as Poria hypobrunnea, Fomes lamaoe...

Endophytic bacteria from selenium-supplemented wheat plants could be useful for plant-growth promotion, biofortification and Gaeumannomyces graminis biocontrol in wheat production
submitted by omanca 2 months ago - Topic: Agriculture
In this study, we isolated putative plant-growth-promoting endophytic bacteria from selenium-supplemented wheat grown under field conditions. These bacterial strains belonged to Bacillus, Paenibacillus, Klebsiella, and Acinetobacter genera and showed genetic similarly with rhizospheric bacteria isolated in the same Andisol soil and with other endophytic strains previously reported. Strains isol...

Diverse and bioactive endophytic Aspergilli inhabit Cupressaceae plant family
submitted by moreonni 1 month ago - Topic: Biology
Aspergilli are filamentous, cosmopolitan and ubiquitous fungi which have significant impact on human, animal and plant welfare worldwide. Due to their extraordinary metabolic diversity, Aspergillus species are used in biotechnology for the production of a vast array of biomolecules. However, little is known about Aspergillus species that are able to adapt an endophytic lifestyle in Cupressaceae...