Biology Agriculture Aquaculture Horticulture Postharvest Forestry Geography Environment Health

First report of Rice orange leaf disease phytoplasma (16 SrI) in rice (Oryza sativa) in India

submitted by nerzor 10 months ago
Rice (Oryza sativa L.,) is one of the most important cereal crops worldwide. In this study, phytoplasmas have been detected in symptomatic leaves of four varieties of rice using nested-PCR with primer pairs P1/P7 and R16F2n/R16R2, which amplified a 1.2 kb fragment of the 16S rRNA gene. The nucleotide sequence analysis of the fragment had 100 % identity among the sequences from the four varieties (GenBank Accession numbers: JX290545, JX290546, JX290547 and JX290548) and 99 % nucleotide sequence identity with 16S rRNA gene sequences of the Rice orange leaf disease phytoplasmas from the Philippines and Thailand, Maize bushy stunt phytoplasma and Wheat blue dwarf phytoplasma belonging to the ‘Candidatus Phytoplasma asteris' (16SrI) group. This is the first report of 16SrI group phytoplasmas infecting rice in India.

Topic: Agriculture

Add your comment

Please Login or Signup to leave a comment

Related stories

Exogenous superoxide dismutase may lose its antidotal ability on rice leaves
submitted by obrawn 1 year and 7 months ago - Topic: Agriculture
Leaf diffusates of the resistant rice (Oryza sativa L.) cultivars suppressed spore germination of blast fungus (Magnaporthe grisea (Hebert) Barr). Bovine Cu-Zn superoxide dismutase (SOD) added to the diffusate abolished its toxicity. However, the enzyme added to the inoculum did not affect the toxicity of the diffusate. Even the second SOD portion added to the diffusate was ineffective. As well...

Salinity tolerance, Na+ exclusion and allele mining of HKT1;5 in Oryza sativa and O. glaberrima: many sources, many genes, one mechanism?
submitted by gseikmon 1 year and 6 months ago - Topic: Agriculture
Cultivated rice species (Oryza sativa L. and O. glaberrima Steud.) are generally considered among the crop species most sensitive to salt stress. A handful of lines are known to be tolerant, and a small number of these have been used extensively as donors in breeding programs. However, these donors use many of the same genes and physiological mechanisms to confer tolerance. Little information i...

Identification of rice sheath blight and blast quantitative trait loci in two different O. sativa/O. nivara advanced backcross populations
submitted by warldcev 1 year and 6 months ago - Topic: Agriculture
Two accessions of Oryza nivara, a wild ancestral species of rice (O. sativa) identified as being moderately resistant to sheath blight and leaf blast disease, were used as donor parents to develop two advanced backcross populations with the US rice cultivar, Bengal, as the recurrent parent. The O. nivara donor parent for Wild-1 (252 BC2F1 lines) was acc. IRGC100898 and for Wild-2 (253 BC2F1 lin...

Observations on the Foliar Nematode, Aphelenchoides besseyi, Infecting Tuberose and Rice in India
submitted by triimon48 1 year and 6 months ago - Topic: Biology
The foliar nematode Aphelenchoides besseyi causes white tip disease in rice (Oryza sativa L.) and floral malady in tuberose (Polianthes tuberosa L.). This nematode is widely distributed in the rice fields of many states of India, including West Bengal (WB), Andhra Pradesh (AP), Madhya Pradesh (MP) and Gujarat (GT). In order to generate information on intraspecific variations of A. besseyi as we...

Overexpression of a BAHD Acyltransferase, OsAt10, Alters Rice Cell Wall Hydroxycinnamic Acid Content and Saccharification
submitted by rmocep 1 year and 5 months ago - Topic: Agriculture
Grass cell wall properties influence food, feed, and biofuel feedstock usage efficiency. The glucuronoarabinoxylan of grass cell walls is esterified with the phenylpropanoid-derived hydroxycinnamic acids ferulic acid (FA) and para-coumaric acid (p-CA). Feruloyl esters undergo oxidative coupling with neighboring phenylpropanoids on glucuronoarabinoxylan and lignin. Examination of rice (Oryza sat...

Novel receptor-like kinases in cacao contain PR-1 extracellular domains
submitted by venad3116 1 year and 5 months ago - Topic: Agriculture
Members of the pathogenesis-related protein 1 (PR-1) family are well-known markers of plant defence responses, forming part of the arsenal of the secreted proteins produced on pathogen recognition. Here, we report the identification of two cacao (Theobroma cacao L.) PR-1s that are fused to transmembrane regions and serine/threonine kinase domains, in a manner characteristic of receptor-like k...

Identification and characterization of OsEBS, a gene involved in enhanced plant biomass and spikelet number in rice
submitted by niaraindacreni 1 year and 1 month ago - Topic: Biology
Common wild rice (Oryza rufipogon Griff.) is an important genetic reservoir for rice improvement. We investigated a quantitative trait locus (QTL), qGP5-1, which is related to plant height, leaf size and panicle architecture, using a set of introgression lines of O. rufipogon in the background of the Indica cultivar Guichao2 (Oryza sativa L.). We cloned and characterized qGP5-1 and confirmed th...

YSL16 Is a Phloem-Localized Transporter of the Copper-Nicotianamine Complex That Is Responsible for Copper Distribution in Rice
submitted by komolkamor 1 year and 10 months ago - Topic: Agriculture
Cu is an essential element for plant growth, but the molecular mechanisms of its distribution and redistribution within the plants are unknown. Here, we report that Yellow stripe-like16 (YSL16) is involved in Cu distribution and redistribution in rice (Oryza sativa). Rice YSL16 was expressed in the roots, leaves, and unelongated nodes at the vegetative growth stage and highly expressed in the u...

Regulation of glutamine synthetase isoforms in two differentially drought-tolerant rice (Oryza sativa L.) cultivars under water deficit conditions
submitted by rechisib 1 year and 10 months ago - Topic: Biology
The regulation of GS isoforms by WD was organ specific. Two GS isoforms i.e. OsGS1;1 and OsGS2 were differentially regulated in IR-64 (drought-sensitive) and Khitish (drought-tolerant) cultivars of rice. Water deficit (WD) has adverse effect on rice (Oryza sativa L.) and acclimation requires essential reactions of primary metabolism to continue. Rice plants utilize ammonium as major nitrogen so...

Pathogenicity of Rice Blast Fungus Magnaporthe oryzae on Brachypodium distachyon
submitted by beagad85 1 year and 11 months ago - Topic: Agriculture
Inoculation methods for rice blast fungus Magnaporthe oryzae to Brachypodium distachyon were developed to investigate the infection process and symptom development in comparison with those on rice (Oryza sativa) and barley (Hordeum vulgare). M. oryzae could infect leaves, sheathes, stems and panicles of B. distachyon and cause blast disease. Spraying conidial suspension on either intact seedlin...

Molecular characterization identifies 16SrXI-B group phytoplasma (‘Candidatus Phytoplasma oryzae’-related strain) associated with root wilt disease of coconut in India
submitted by bab2 9 months ago - Topic: Agriculture
The root wilt disease of coconut is a major threat to coconut cultivation in southern India. Here we report the species assignment and 16Sr sub group classification of phytoplasma associated with coconut root wilt disease. Leaf samples were collected from root wilt symptomatic palms in Kayankulam district of Kerala. The phytoplasma 16S rRNA gene was amplified using three sets of primers namely,...

Rice yellow dwarf phytoplasma (16Sr XI-B subgroup) infecting Jasminum sambac in India
submitted by kawhishe 1 month ago - Topic: Agriculture
Leaf yellowing and witches’ broom symptoms were recorded on Jasminum sambac (L.) plants in August 2012 at Jaipur, Rajasthan, India. Total DNA was isolated from symptomatic jasmine plants and nested PCR was performed using primers pairs P1/P7 and 3Far/3Rev. Expected size amplicons of ~1.3 kb were obtained in nested PCR from symptomatic jasmine plants which were cloned and sequenced. BLASTn ana...