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Detection of the Dengue Virus NS1 Antigen Using an Enzyme Immunoassay

submitted by beggybaanty 5 months ago
Current diagnostic methods for dengue virus (DV) rely primarily on detection of anti-DV antibodies and/or DV RNA by reverse transcriptase (RT) PCR. Several limitations exist however: seroconversion is delayed following infection and DV RT-PCR assays are not yet readily available. The DV NS1 antigen is an alternative acute phase DV biomarker and here we evaluated the new InBios DENV DetectTM NS1 ELISA compared to DV RT-PCR and serology for detection of recent DV infection. We report a positive, negative and overall percent agreement of 96% (24/25), 86.0% (43/50) and 89.3% (67/75) for the InBios NS1 ELISA compared to DV RT-PCR. Performance of the NS1 ELISA compared to serology for anti-DV IgM antibodies showed a positive, negative, and overall percent agreement of 78.0% (85/109), 90.7% (333/367) and 87.8% (418/476), respectively. Collectively, the InBios NS1 ELISA can be used as an alternative to DV RT-PCR for identification of acute DV infection.

Topic: Biology

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